Abstract
Naturally transgenic plant species occur on an unexpectedly large scale. Agrobacterium-mediated gene transfer leads to the formation of crown galls or hairy roots, due to expression of transferred T-DNA genes. Spontaneous regeneration of transformed cells can produce natural transformants carrying cellular T-DNA (cT-DNA) sequences of bacterial origin. This particular type of horizontal gene transfer (HGT) could play a role in plant evolution. However, the material available today is not enough for generalizations concerning the role of Agrobacterium in HGT from bacteria to plants. In this study, we searched for T-DNA-like genes in the sequenced genomes of dicots and monocots. We demonstrate the presence of cT-DNAs in 23 out of 275 dicot species, within genera Eutrema, Arachis, Nissolia, Quillaja, Euphorbia, Parasponia, Trema, Humulus, Psidium, Eugenia, Juglans, Azadirachta, Silene, Dianthus, Vaccinium, Camellia, and Cuscuta. Analysis of transcriptome data of 356 dicot species yielded 16 additional naturally transgenic species. Thus, HGT from Agrobacterium to dicots is remarkably widespread. Opine synthesis genes are most frequent, followed by plast genes. Species in the genera Parasponia, Trema, Camellia, Azadirachta, Quillaja, and Diospyros contain a combination of plast and opine genes. Some are intact and expressed, but the majority have internal stop codons. Among the sequenced monocot species, Dioscorea alata (greater yam) and Musa acuminata (banana) also contain T-DNA-like sequences. The identified examples are valuable material for future research on the role of Agrobacterium-derived genes in plant evolution, for investigations on Agrobacterium strain diversity, and for studies on the function and evolution of cT-DNA genes in natural transformants.
Keywords: Horizontal gene transfer; Naturally transgenic plants; Transcriptome shotgun assembly; Whole-genome shotgun contigs; cT-DNA.
Tatiana V Matveeva , Léon Otten Plant Mol Biol, 101 (4-5), 415-437 Nov 2019
Similar articles
The Genome of Cultivated Sweet Potato Contains Agrobacterium T-DNAs With Expressed Genes: An Example of a Naturally Transgenic Food CropT Kyndt et al. Proc Natl Acad Sci U S A 112 (18), 5844-9. 2015. PMID 25902487.
Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes …
Agrobacterium-Mediated Transformation in the Evolution of Plants
TV Matveeva. Curr Top Microbiol Immunol 418, 421-441. 2018. PMID 29500560. - Review
In most cases, the genetic engineering of plants uses Agrobacterium-mediated transformation to introduce novel genes. In nature, insertion of T-DNA into the plant genome …
The Agrobacterium Phenotypic Plasticity (Plast) Genes
L Otten. Curr Top Microbiol Immunol 418, 375-419. 2018. PMID 29770865. - Review
The transfer of T-DNA sequences from Agrobacterium to plant cells is a well-understood process of natural genetic engineering. The expression of T-DNA genes in plants lea …
Horizontal Gene Transfer Contributes to Plant Evolution: The Case of Agrobacterium T-DNAs
DG Quispe-Huamanquispe et al. Front Plant Sci 8, 2015. 2017. PMID 29225610. - Review
Horizontal gene transfer (HGT) can be defined as the acquisition of genetic material from another organism without being its offspring. HGT is common in the microbial wor …
Horizontal Gene Transfer From Genus Agrobacterium to the Plant Linaria in Nature
TV Matveeva et al. Mol Plant Microbe Interact 25 (12), 1542-51. Dec 2012. PMID 23134518.
Genes can be transferred horizontally between prokaryotes and eukaryotes in nature. The best-studied examples occur between Agrobacterium rhizogenes and certain Nicotiana …
No comments:
Post a Comment